FIGURE

Fig. 5-S1

ID

ZDB-FIG-180724-12

Publication

Janjuha et al., 2018 - Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish

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Fig. 5-S1

The GFP fluorescence of the transgenic reporterTg(NF-kB:GFP)remains stable for 72 hours in beta-cells.

(a,b,c) Beta-cells from Tg(NF-kB:GFP);Tg(ins:mCherry) animals at 3 mpf were FAC-sorted as single cells in 384-well plates and followed over 72 hr. NF-kB:GFP^high cells at 24, 48 and 72 hr post-FAC-sorting. Scale bar 5 µm. (a’,b’,c’) NF-kB:GFP^low cells at 24, 48 and 72 hr post-FAC-sorting. Scale bar 5 µm. (d) Quantification showing the mean GFP fluorescence intensity of GFP^high and GFP^low cells (n = 7 GFP^high cells and n = 5 GFP^low cells).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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