Fig. S3
- ID
- ZDB-FIG-180720-40
- Publication
- Löhr et al., 2018 - Diet-Induced Growth Is Regulated via Acquired Leptin Resistance and Engages a Pomc-Somatostatin-Growth Hormone Circuit
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The sst1.1:EGFPras transgenic line recapitulates endogenous sst1.1 expression and reveals activation of hypophysiotropic Sst1.1 neurons after ?MSH ICV injection. Related to Figures 5 and 7. (A-B??) sst1.1 FISH (magenta) in combination with GFP IF (green) in sst1.1:EGFPras transgenic fish at 15 dpf (A-A??) and 42 dpf (B-B??) revealing co-localization of sst1.1 transcripts and GFP both in the anterior (a) and posterior (p) domains of the preoptic area (POA). (C-D??) Co-IF for GFP (green) and pERK (magenta) on cross-sections (12 ?m) at the level of posterior Sst1.1 POA cell clusters of tg(sst1.1:EGFPras) fish (42 dpf), 30 min after cerebroventricular injection of saline (C-C??) or ?MSH/saline (DD??). pERK levels are strongly increased after ?MSH application in a broad region of the POA including Sst1.1 neurons (see arrowheads and inset in D??). Scale bars: (A??): 50 ?m, (B??, C??) 100 ?m. ac: anterior commissure, poc, postoptic commissure. |