Fig. 7
- ID
- ZDB-FIG-180720-37
- Publication
- Löhr et al., 2018 - Diet-Induced Growth Is Regulated via Acquired Leptin Resistance and Engages a Pomc-Somatostatin-Growth Hormone Circuit
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Zebrafish Sst Neurons Respond to ?MSH to Mediate ?MSH?s Effect on GH Expression (A?G) pERK response of hypophysiotropic Sst1.1 cells to ?MSH treatment in sst1.1:EGFPras fish (42 dpf). (A?B??) POA of two hemispheres derived from the same brain, differentially incubated in saline (A?A??) or 10 ?M ?MSH (B?B??) for 30 min. Double IF for GFP (green) and pERK (magenta) reveals increase in pERK levels after ?MSH application in the posterior POA (p). Images of the right hemisphere were flipped horizontally. (C?D??) Magnification of posterior POA Sst1.1 neurons without (C?C??) or with (D?D??) ?MSH treatment. (E?G) Quantification of pERK (E and F) and GFP (E and G) levels in a total of seven brain hemisphere pairs as shown in (A) and (B), revealing significantly increased pERK, but unaltered GFP levels in posterior Sst1.1 neurons after ?MSH treatment. (H) Effect of long-term ?MSH or SHU9119 treatment on sst1.1 mRNA levels. sst1.1 FISH and signal intensity determination from confocal images of juvenile fish (42 dpf) that had been subjected to three repetitive ICV injections of ?MSH, SHU9119, or saline over the course of 48 hr. (I) Epistasis between Pomca/?MSH and Sst systems; gh1 expression levels assessed by qRT-PCR analysis after treatment of larvae (14 dpf) for 8 hr with 10 ?M ?MSH, 1 ?M cSST, or a combination of both peptides. Scale bars represent 50 ?m (A?B??) and 10 ?m (C?D??). ?p < 0.05; ??p < 0.01; ???p < 0.001. Error bars in (F)?(I) show SD. |