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ZFIN ID: ZDB-FIG-180711-19
Bouffard et al., 2018 - Fibrillarin is essential for S-phase progression and neuronal differentiation in zebrafish dorsal midbrain and retina. Developmental Biology   437(1):1-16 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 2

Gross morphological defects in fbl null mutants are mainly seen in the head and eyes and are rescued by injection of fbl mRNA. (A) RT-qPCR quantification of relative levels of fbl mRNA at the indicated developmental stages shows the absence of fbl expression in mutants as early as the 24 hpf stage. Purple: wild-type, gray: siblings, pink: mutants. Statistical analyses were performed on four biological samples per condition. p-value (Kruskal-Wallis test) polysomes: 0.02. n.s., non-significant. (B1-B6) Lateral views of live embryos with anterior to the left and dorsal up. (B1-B2)fblhi2581Tg mutants start to display phenotypic abnormalities as early as the 24 hpf stage with necrosis in the midbrain area, slightly smaller eyes and a thin yolk extension. (B3-B4) At 48 hpf, mutant embryos have smaller eyes and smaller head than their siblings. In addition, mutant embryos display less pigmentation, pericardiac edema, a larger and rounder yolk and a thinner yolk extension. (B5-B6) At 48 hpf, fbl mutant embryos are largely rescued by fbl mRNA. In injected mutants, midbrain and yolk extension look similar in size to the ones in siblings. The size of the eye is bigger than in not injected mutants although remaining slightly smaller than in siblings. In panels B1-B9, eyes, midbrain area and yolk extension are respectively indicated by arrowheads, arrows and asterisks. Scale bar = 200 µm. (C) Classes of phenotypes of fbl mRNA injected embryos from an fblhi2581Tg incross. A strong decrease in the number of injected embryos with mutant phenotype is observed indicating that the fbl mRNA rescues the mutation.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fbl fblhi2581Tg/hi2581Tg standard conditions Prim-5 Not Detected whole organism RTPCR
Long-pec Not Detected whole organism RTPCR
Protruding-mouth Not Detected whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fblhi2581Tg/hi2581Tg standard conditions Prim-5 extension decreased thickness, abnormal
Prim-5 eye decreased size, abnormal
Prim-5 midbrain anatomical region necrotic, abnormal
Prim-5 whole organism fbl expression absent, abnormal
Long-pec ball increased size, abnormal
Long-pec ball shape, abnormal
Long-pec extension decreased thickness, abnormal
Long-pec eye decreased size, abnormal
Long-pec head decreased size, abnormal
Long-pec pericardium edematous, abnormal
Long-pec whole organism fbl expression absent, abnormal
Long-pec whole organism decreased pigmentation, abnormal
Protruding-mouth whole organism fbl expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 437(1), Bouffard, S., Dambroise, E., Brombin, A., Lempereur, S., Hatin, I., Simion, M., Corre, R., Bourrat, F., Joly, J.S., Jamen, F., Fibrillarin is essential for S-phase progression and neuronal differentiation in zebrafish dorsal midbrain and retina, 1-16, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.