Fig. 4

shank3b ^−/− adult zebrafish displayed reduced and repetitive locomotion activity in the open-field test. a Schematic diagram of the open-field test and thigmotaxis test of adult male zebrafish. In the analysis of thigmotaxis test, the area of the peripheral zone is equal to the center zone (dotted line). b–c shank3b^−/− zebrafish at 3.5 mpf showed significantly reduced velocity in the total 60 min period (b) and velocity per larva (c) in the open field (N = 13 for each group). d Representative traces of individual shank3b ^+/+ or shank3 ^−/− zebrafish in the thigmotaxis test. e Ratio for the time spent and distance traveled (periphery divided by the total zone) over 30 min in adult male zebrafish (3.5 mpf). N = 13 for each group. f–g Representative trace of different types of stereotyped behaviors of shank3b ^−/− adult male zebrafish (3.5 mpf). shank3b^−/− zebrafish had a significantly higher proportion of figure “8” and big circling movements than shank3b ^+/+ . N = 13 for each group. Data are shown as mean ± SEM; **p < 0.01, ****p < 0.0001