Fig. 4

Dopaminergic innervation of the anterior lateral line neuromasts. (A?E) Panels 1: schematic showing location of projections (green) and neuromasts (circles) relative to anatomical landmarks. Panels 2 and 5 (green): th:Gal4 driven eGFP-CAAX and SypGFP (GFP) expression visualized by immunofluorescence. Panels 3 and 5 (magenta): anti-TH immunofluorescene. Panels 4 and 5 (blue): TOTO-3 labeling of cell nuclei. MIP of confocal stacks, step size (A?E) 1 ?m, scale bars: 50 ?m. (A). Dorsal view of right half of larval head (total depth of 135 ?m) containing neuromasts (NM) of the otic (O) and supraorbital branch (SO). Arrowheads point to NM SO1?SO3 and O1. (B). Right lateral part of head, dorsal view of DA projections to NM SO3 and O1 and O2. Also visible, ventrally located NM IO4 (asterisk). Total depth of 122 ?m. (C). Right frontal part of head, dorsal view of DA projection to nasal NM (N), total depth of 122 ?m. (D). Right lateral part of head ventral view of DA projection to NM of the infraorbital branch (IO2 and IO3) and mandibular NM (M), total depth of 91 ?m. (E). Right lateral part of head ventral view of DA projection to NM of the infraorbital branch (IO), mandibular (M), nasal (N), and opercular branch (OP). Also visible, the medial NM MI2. Total depth of 226 ?m.