FIGURE

Fig. 2

ID

ZDB-FIG-180703-72

Publication

Knickmeyer et al., 2018 - TGFβ-facilitated optic fissure fusion and the role of bone morphogenetic protein antagonism

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Fig. 2

TGFβ in the zebrafish eye. (a) Whole-mount in situ hybridization (WMISH) of tgfb2 (30 hpf), frontal view. Small image shows a lateral view. Note expression in periocular tissue (arrowheads). (b) WMISH of tgfb3 (30 hpf), lateral view. Note expression in the developing lens. (c) WMISH of tgfbr2 (30 hpf), lateral view. Note expression in the optic fissure (arrow). (d) In vivo signalling for TGFβ (green) and Shh (red) for orientation, split into TGFβ (d′) and Shh (d″), at 24 hpf. Active TGFβ signalling in the optic fissure margins (d′, arrows). (e–j) Embryos (3 dpf) treated with DMSO (e–g) or specific inhibitor of Smad3 (SIS3, h–j) from 24 to 30 hpf. Sagittal sections through eyes of (g) DMSO and (j) SIS3-treated embryos at 3 dpf, stained with DAPI and anti-Laminin. SIS3-treated embryos show a persisting optic fissure with persisting basal lamina and absence of retinal layering.

Expression Data

Genes:	GFP mCherry tgfb2 tgfb3 tgfbr2b
Antibody:	Ab1-lam
Fish:	AB af2Tg; af3Tg
Condition:	chemical treatment by environment: SIS3
Anatomical Terms:	eye basement membrane lens optic fissure anatomical margin optic furrow optic furrow basement membrane optic stalk periocular mesenchyme
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment by environment: SIS3
Observed In:	brain brain development closure of optic fissure eye optic fissure optic furrow basement membrane post-vent region
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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