FIGURE

Fig. S3

ID

ZDB-FIG-180703-19

Publication

Zhou et al., 2018 - Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish

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Fig. S3

Decreasing the ROS level in neutrophils does not affect neutrophil migration.

A, B) Tg (lyzC:GFP) at 3 dpf was treated with DMSO, DPI, Celastrol or NAC. A) Representative images and B) Quantification of neutrophil motility in the head mesenchyme. One representative result of three biological repeats was shown. n = 32 for DMSO, n = 32 for DPI, n = 33 for Celastrol, n = 24 for NAC from 4 different larvae. n.s., not significant by one-way ANOVA. C) Representative images and D) Quantification of neutrophil number at the wound at 1 hpw in embryos treated with DMSO, DPI, or Celastrol. One representative result of three biological repeats was shown. n = 19 for DMSO, n = 21 for DPI, n = 17 for Celastrol. ****, p<0.0001 by Kruskal-Wallis test. E) Representative images and F) Quantification of neutrophil motility in the head mesenchyme with neutrophil specific transient overexpression of Sod2. n = 28 for ctrl and n = 27 for Sod2-2A-Catalase from 4 different larvae. n.s., not significant by Mann-Whitney test. Scale bars: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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