Fig. 4

Mitochondria redox status regulates neutrophil motility. (A,B) Representative images (A) and quantification (B) of neutrophil motility in the head mesenchyme with neutrophil-specific transient knockout of sod1 and sod2 using the plasmids described in Fig. 3C. One representative result from three biological repeats is shown. n=36 for control (ctrl), and n=40 for sod1 and n=42 for sod2 knockouts from four different larvae. ****P<0.0001 by Kruskal–Wallis test. (C,D) Representative images (C) and quantification (D) of neutrophil motility in embryos co-injected with the plasmid for sod1 knockout in neutrophils and sod1 mRNA or the GFP control mRNA. One representative result from three biological repeats is shown. n=11 for gfp mRNA and n=14 for sod1 mRNA from four different larvae. **P<0.01 by Mann–Whitney test. (E,F) Representative images (E) and quantification (F) of neutrophil motility in embryos injected with the plasmid for sod1 knockout in neutrophils and then treated with 1% DMSO, 200 µM NAC or 50 µM mitoTEMPO from 1 dpf. One representative result from three biological repeats is shown. n=53 for DMSO, n=34 for NAC and n=62 for mitoTEMPO from four different larvae. ****P<0.0001 by Kruskal–Wallis test. (G,H) Representative images (G) and quantification (H) of neutrophil motility in embryos injected with the plasmid for sod2 knockout in neutrophils and then treated with 1% DMSO, 200 µM NAC or 50 µM mitoTEMPO from 1 dpf. One representative result from three biological repeats is shown. n=49 for DMSO, n=40 for NAC and n=31 for mitoTEMPO from four different larvae. **P<0.01; n.s., not significant, by Kruskal–Wallis test. Scale bars: 50 µm.