Recruitment of peripheral blood monocytes in granuloma formation.
(A-C) Granuloma formation in Tg(mfap4:tdTomato) fish with red-fluorescent macrophages and infected with green-fluorescent M. marinum. Hoechst-positive (blue-fluorescent) peripheral blood monocytes which have been recruited into the infected eye are seen within the granuloma (inset and panel B) and in contact with a single infected macrophage (inset and panel C). The retinal microglia are incorporated into the granuloma and also dispersed uniformly within the ocular tissues. Scale bars, 30 μm.