FIGURE

Fig. 1

ID

ZDB-FIG-180702-19

Publication

Bergboer et al., 2018 - Assaying sensory ciliopathies using calcium biosensor expression in zebrafish ciliated olfactory neurons

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Olfactory epithelium (OE) in 2.5-dpf zebrafish. Multi-ciliated motile cilia are present at the border, ciliated OSNs in the centre; maximum intensity projection. a Basal bodies stained with anti-cep290 (red), a’ cilia stained with anti-acetylated tubulin (green), a” merged image. Inset in a” closeup of sensory (left) and motile (right) cilia showing basal body staining of anti-Cep290. b EM image of dendritic knob of ciliated zebrafish OSNs containing multiple basal bodies (arrowheads). c Tg(omp:mCherry,trpc2:Venus) zebrafish with ciliated Omp-positive OSNs (red) and c’ microvillus TrpC2-positive OSNs (green), c” merged image; single confocal section. d Quantification of C, N = 5 fish. e Tg(omp:mCherry,elavl3:GCaMP5) zebrafish show overlap between ciliated Omp-positive OSNs (red) and e’ GCaMP5-positive OSNs (green) OSNs, e” merged single confocal section. f Quantification of E, N = 6 fish. Bars represent mean and SEM. Scale bar is 10 µm, except b bar is 1 µm

Expression Data

Genes:	GCaMP mCherry Venus
Antibodies:	Ab1-tuba Ab2-cep290
Fish:	WT rw037Tg/rw037Tg; zf2143Tg/zf2143Tg
Anatomical Terms:	ciliated olfactory receptor neuron ciliated olfactory receptor neuron cilium
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cilia