Fig. 1
The Notochord Sheath Displays a Segmented Pattern Prior to Vertebral Body Formation (A) Live confocal imaging of notochord segmentation (denoted by brackets) and osteoblast recruitment (arrows) in col9a2:GFPCaaX and osx:mcherry-NTR fish. (B) Live confocal imaging showing that osteoblasts specifically migrate to col9a2-negative domains (brackets) in an anteroposterior manner. (C) Live imaging of calcein stained col9a2:mcherry fish showing that col9a2-negative domains (denoted by asterisks) become mineralized. Developmental stages are based on standard length. All scale bars are 100 μm. Images in (A) and (C) are digitally stitched. |