Fig. 3

Pontin is a cytoplasmic protein dispensable for cilia morphogenesis. (A) Pontin is enriched in cytoplasmic puncta in the pronephric tubule (outlined) of wild-type zebrafish embryos, but is reduced in pontin^{hi1055B/hi1055B} mutants (MUT) at 2 dpf. Cilia are labeled with anti-acetylated Tubulin (A-Tub; red), Pontin is immunostained in green and nuclei are stained with DAPI (blue). MCC, multiciliated cells. (B) Pontin is enriched in cytoplasmic puncta in the olfactory placode (OLF) and the lateral line organ (LL) at 60 hpf. Five embryos were observed. (C) Cilia bundles (arrows) from multiciliated cells in the pronephric tubule, the olfactory placode at 5 dpf and the lateral line organ at 4 dpf in wild type and pontin^{hi1055B/hi1055B} mutants. SCC, single-ciliated cells. Cilia are labeled with anti-acetylated Tubulin (green). Pronephric epithelial cells are labeled with anti-Cdh17 (red). F-actin in hair cells of the lateral line organ is labeled with phalloidin (red). (D) Still images captured from live Nomarski videos showing abundant cilia in the olfactory placode at 3 dpf in both wild type and pontin^{hi1055B/hi1055B} mutants. Dashed line indicates the distal tip of numerous cilia. 10 wild-type and 9 pontin^{hi1055B/hi1055B} mutant embryos were observed.