FIGURE

Fig. 2

ID

ZDB-FIG-180502-2

Publication

Li et al., 2017 - Axonemal dynein assembly requires the R2TP complex component Pontin

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Fig. 2

Pontin and Reptin function together in zebrafish development. (A) pontin^{hi1055B/hi1055B} (pon), reptin^{hi2394/hi2394} (rep) and double mutants at 4 dpf show ventral body curvature and kidney cysts (arrows), as compared with a wild-type embryo. A total of 247 embryos were inspected. (B) Pontin and Reptin depend on each other for protein stability. Total lysates of reptin^{hi2394/hi2394} (rep, MUT), pontin^{hi1055B/hi1055B} (pon, MUT) and respective control siblings (C) at 4?dpf were subject to IP and blotted with anti-Reptin (Rep) or anti-Pontin (Pon). Western blot of ?-Tubulin (Tub) on total lysates was used as a loading control. 20 embryos were used for each sample. The results shown are representative of two independent experiments.

Expression Data

Genes:	ruvbl1 ruvbl2
Antibodies:	Ab1-ruvbl1 Ab1-ruvbl2
Fish:	AB/TU ruvbl1^{hi1055bTg/hi1055bTg} (AB/TU) ruvbl2^{hi2394Tg/hi2394Tg} (AB/TU)
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ruvbl1^{hi1055bTg/hi1055bTg} (AB/TU) ruvbl2^{hi2394Tg/hi2394Tg} (AB/TU) ruvbl1^{hi1055bTg/hi1055bTg}; ruvbl2^{hi2394Tg/hi2394Tg} (AB/TU)
Observed In:	post-vent region pronephros trunk whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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