|ZFIN ID: ZDB-FIG-180426-2|
Fig. 3 otx5 regulates expression of lypge. (A) Two kinds of otx5 binding sequence “TAATC” and “TAATT” are marked by blue and red, respectively, in promoter (− 1920 to − 1) and downstream sequence (7601 to 11,143) of lypge. (B) Ectopic expression (arrowhead) is induced in otx5 overexpressed embryos at 24 hpf, pineal gland is indicated by arrow. (C) Left: examination of otx5 MO efficiency, expression of EGFP fluorescence in embryos at 10 hpf is absent from embryos co-injected MO-binding site + EGFP mRNA with otx5 MO. Pineal expression of lypge in wildtype and otx5 MO emryos at 24 hpf. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
|ly6pge||WT + MO1-otx5||standard conditions||Prim-5||Not Detected||epiphysis||ISH|
|WT + MO1-otx5||standard conditions||Prim-5||epiphysis ly6pge expression absent, abnormal|
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Reprinted from Gene, 642, Ji, D., Wang, S., Li, M., Zhang, S., Li, H., Involvement of Lypge in the formation of eye and pineal gland in zebrafish, 491-497, Copyright (2017) with permission from Elsevier. Full text @ Gene