FIGURE

Fig. S9

ID

ZDB-FIG-180425-7

Publication

Moreno-Mateos et al., 2017 - CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

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Fig. S9

Longer incubations at 34°C improve AsCpf1 activity in vivo.

a. A representative picture showing 48 hpf old embryos obtained after AsCpf1-crRNA RNP complexes injections targeting tyr in the conditions described in Fig. 2f. Scale bar, 1.25 mm.

b. Phenotypic evaluation of AsCpf1-crRNA (left) or AsCpf1-pre-crRNA (right) RNP complexes (10 fmol) injections targeting slc45a2 (alb) in the conditions described in Fig. 2f. Stacked barplots showing the percentage of mosaic mutants (grey), and phenotypically WT (black) embryos 48 hpf after injection. Number of embryos evaluated (n) is shown for each condition. ?² test (****p< 0.0001).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.