ZFIN Image: Moreno-Mateos et al., 2017, Fig. S9

Image

Figure Caption

Fig. S9

Longer incubations at 34�C improve AsCpf1 activity in vivo.

a. A representative picture showing 48 hpf old embryos obtained after AsCpf1-crRNA RNP complexes injections targeting tyr in the conditions described in Fig. 2f. Scale bar, 1.25 mm.

b. Phenotypic evaluation of AsCpf1-crRNA (left) or AsCpf1-pre-crRNA (right) RNP complexes (10 fmol) injections targeting slc45a2 (alb) in the conditions described in Fig. 2f. Stacked barplots showing the percentage of mosaic mutants (grey), and phenotypically WT (black) embryos 48 hpf after injection. Number of embryos evaluated (n) is shown for each condition. ?² test (****p< 0.0001).

Acknowledgments

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