Fig. 2

H2afv depletion causes significant phenotypic abnormality (A) Bright field images comparing Control (uninjected) and h2afv MO injected fish at 24 and 48 hpf, with the same embryo imaged at both time points. (B) Embryos were scored as Severe, Mild or Normal phenotype in a minimum of 5 clutches and displayed as percentages, with n = the number of total embryos and the number clutches analyzed per condition. (C) Western blot analysis of 24 hpf embryos demonstrates complete depletion of the H2afv protein in morphants exhibiting the severe phenotype and slight residual protein expression in h2afz morphants with mild phenotype while the control (uninjected) embryos express high levels of H2afv protein. (D) Bright field images of 48 hpf embryos injected with h2afv mRNA (50 ng), h2afv MO or h2afv mRNA plus h2afz MO. Phenotypic normality is restored in co-injected embryos. (E) Phenotype percentages of h2afv MO or h2afv mRNA injected with control morpholino-­1 or with h2afz morpholino scored at 48 hpf. Scale bar: 1000 μm.