Fig. 5

Development of lethal mutant embryos and larvae grafted into rag1 mutants. (a,c) Morphology of 72?hpf spns2 mutant embryos (a) and 7?dpf oep mutant larvae (c) before and after transplantation into germ cell-depleted rag1 mutants. The spns2 mutant has cardia bifida (two hearts) as determined by cmlc2-driven mRFP expression (arrowheads), and the oep mutant exhibits a single eye (arrows). Scale bar: 1?mm. (b,d) Histology of organs found in grafted spns2 mutant embryos (b) and oep mutant larvae (d) at 2 months after transplantation. Arrowheads indicate each organ as labelled above the panels. Note that testes of the grafted spns2 and oep mutants contained all stages of spermatogenic cells: spermatogonia (g), spermatocytes (c), and sperm (s). The heart was not observed in grafted spns2 mutant embryos (b). Scale bar: 200?µm. (e) RT-PCR analysis of grafted spns2 and oep mutants at 2 months post-transplantation. Wild-type zebrafish at 2 mpf were used as a positive control. Negative controls lacking reverse transcriptase are in alternating lanes (?). Each experiment was conducted using two independent samples.