Fig. 2

hace1?morphant embryos display specific cardiac phenotypes that can be rescued by human HACE1 mRNA while the vasculature remains intact. A: The looping defect that results from hace1 knockdown results in either misalignment of the atrium and the ventricle (the ventricle is located on the left side [white arrow], ?inverted?) in ii or a tubular heart (?straight') in iii. Scale bar?=?250?µm. B: Diagrammatic representation of each phenotype is included adjacent to a fluorescent image of a Tg(myl7::eGFP) hace1?morphant embryo with the associated phenotype. C: Bar graphs showing the percent of each of the representative anatomic phenotypes shown in panels A and B (colors correspond to the respective phenotypes; n?=?90?130 embryos for each group in 3 replicates; lateral views at 48 hpf; **P??0.05). Scale bar?=?250?µm.