Fig. 3

Whole‐mount confocal imaging and in situ hybridization of the stable Tg(mustn1b:eGFP)iim01 line embryos and larvae. A: Expression of eGFP reporter gene in muscle pioneer cells and somites in 30‐hpf transgenic embryos. The white arrowhead indicates the strong eGFP expression in a marked stripe of muscle pioneer cells adjacent to the notochord. A1–A4: Confocal imaging of cross‐sections from 30‐hpf transgenic embryo. B: Whole‐mount in situ hybridization showing expression of mustn1b mRNA in muscle pioneer cells and somites in 30‐hpf embryos. The black arrowhead indicates mustn1b gene expression in the stripe of muscle pioneer cells adjacent to the notochord. B1–B4: Cross‐sections from 30‐hpf embryo with in situ hybridization staining. C–F: eGFP expression in mononucleated skeletal and heart muscles of transgenic Zebrafish at 2, 4, and 7 dpf. E,G: eGFP expression in craniofacial and fin muscles of transgenic larvae at 4 and 7 dpf. e, eye; am, adductor mandibularis; hh, hyohyal; ih, interhyal; ima, intermandibularis anterior; imp, intermandibularis posterior; io, inferior oblique; ir, inferior rectus; sh, sternohyoideus; lap, levator arcus palatine; ah, adductor hyoideus; ao, adductor operculae; do, dilator operculae; lo, levator operculae; bl5, 5th branchial levator; bl1‐4, 1st–4th branquial levators; tv, transversus ventralis; pfm, pectoral fin muscles. Scale bars A,B,C,E = 200 μm. Scale bars A1–A4 = 50 μm. Scale bars B1–B4 = 25 μm. Scale bars D,F,G = 100 μm.