Fig. 3

The proliferation of xenografted C4-2 CRPC cells in vivo is not affected by testosterone or enzalutamide. (A) Representative brightfield and fluorescent images of casper embryos injected with CMTMR labeled C4-2 cells at 24 hpi (baseline) and 96 hpi (endpoint) without and with the addition of testosterone (125 nM), enzalutamide (5 µM) or combined treatment. Scale bar = 200 microns. (B) Quantification of fluorescently labeled XT C4-2 cells showed that the addition of 5 µM of enzalutamide (ENZ), 125 nM of testosterone (T) or a combination of both compounds had no significant effect on cell growth in the zebrafish model. Error Bars = Mean ± SEM (N = 4); *P < 0.05 for significant differences in cell numbers determined using the Student’s t-test. Groups of 20 embryos were sacrificed per replicate.