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Fig. 3
The proliferation of xenografted C4-2 CRPC cells in vivo is not affected by testosterone or enzalutamide. (A) Representative brightfield and fluorescent images of casper embryos injected with CMTMR labeled C4-2 cells at 24 hpi (baseline) and 96 hpi (endpoint) without and with the addition of testosterone (125?nM), enzalutamide (5?µM) or combined treatment. Scale bar?=?200 microns. (B) Quantification of fluorescently labeled XT C4-2 cells showed that the addition of 5?µM of enzalutamide (ENZ), 125?nM of testosterone (T) or a combination of both compounds had no significant effect on cell growth in the zebrafish model. Error Bars?=?Mean?±?SEM (N?=?4); *P?<?0.05 for significant differences in cell numbers determined using the Student?s t-test. Groups of 20 embryos were sacrificed per replicate.