FIGURE

Fig. 2

ID

ZDB-FIG-180321-3

Publication

Hozumi et al., 2017 - Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification

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Fig. 2

Overexpression of ndr1 mRNA in the YSL leads to nuclear movement in MMCs toward the YSL. (A) Experimental design for ndr1 mRNA overexpression in YSL (ndr1 mRNA>YSL). (B-C′) Transverse sections of WT and ndr1 mRNA>YSL embryos; the cell membrane, nucleus and sox32 mRNA were visualized by β-cat staining, DAPI staining and FISH, respectively at 4.7 hpf. Arrows indicate MMCs. Brackets indicate the fluorescence of dextran-Alexa Fluor 594 in the YSL. Both nuclear movement and sox32 expression in MMCs were induced by ndr1 mRNA>YSL at 4.7 hpf. Scale bars: 10 µm. (D,E) DNB and DCN in MMCs were measured in WT and ndr1 mRNA>YSL embryos. ***P<0.001, **P<0.05. (F) Angles between the center of the cell and the nucleus relative to the medial-lateral axis were measured in WT and ndr1 mRNA>YSL embryos as shown in Fig. S1. n, number of nuclei examined (D-F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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