FIGURE

Fig. 4

ID

ZDB-FIG-180321-15

Publication

Campbell et al., 2017 - Phosphodiesterase Inhibitors Sildenafil and Vardenafil Reduce Zebrafish Rod Photoreceptor Outer Segment Shedding

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Fig. 4

Sildenafil and vardenafil reduce adult rod outer segment shedding. (A) Schematic timeline for examining rod outer segment renewal in adult, 6 days post heat shock (dpHS) Tg(Xla.rho:EGFP); Tg(hsp70:HA-mCherryTM); albb4/b4 fish after bathing in phosphodiesterase inhibitor. (B) Representative images of photoreceptor layer from inhibitor-bathed adults immunolabeled with anti-GFP (green), anti-HA (red), and anti-Rhodopsin (blue) antibodies. Images are projections of a subset of z-sections totaling 5.97 μm. Scale bar: 10 μm. Quantification of (C) distance to shed (DS) and (D) growth distance (DG) in inhibitor-bathed adults. Lower and upper hinges of box correspond to first and third quartiles; middle corresponds to median; whiskers extend 1.5 * interquartile range above and below the hinges; dots represent outliers. Graphs represent (C) n = 3 fish/treatment (123 DMSO, 102 sildenafil, 133 vardenafil outer segments) and (D) n = 3 fish/treatment (176 DMSO, 192 sildenafil, 171 vardenafil outer segments). *95% CI of difference compared to control does not span zero.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.