Fig. s4

OTG acts upstream of β-catenin and tcf3/hdl in Wnt signaling pathway

(A-C) Top view, dorsal is to the right. Expression pattern of chd in control (A, 10/10, 100%), wnt8 mRNA-injected (B, 8/10, 80%), or wnt8 and OTG mRNA-injected (C, 10/12, 83%) embryo at sphere stage (4 hpf). Wnt8-induced ectopic expression of chd was inhibited by OTG overexpression. (D-F') Morphology and expression pattern of shh (insets) in control (D, 10/10, 100%; D'), β- catenin mRNA-injected (E, 12/15, 80%; E'), or β-catenin and OTG mRNA-injected (F, 13/14, 93%; F') embryo at 30 hpf and 6 ss. OTG overexpression does not inhibit β-catenin-induced ectopic eye formation or secondary axis formation marked by the ectopic expression of shh. (G-I) Morphology of control (G, 10/10, 100%), VP16-tcf3 (a dominant negative form of tcf3) mRNA-injected (H, 14/18, 78%), or VP16-tcf3 and OTG mRNA-injected (I, 16/18, 89%) embryo at 30 hpf. OTG overexpression does not rescue VP16-tcf3-induced headless-like phenotype. (J-L) Morphology of control (J, 10/10, 100%), SB415286 treated (K, 14/15, 93%), or SB415286 treated and OTG mRNA-injected embryo (L, 14/18, 78%) at 28 hpf. Injection of OTG mRNA does not rescue SB415286-induced headless-like phenotype.