Fig. 1
- ID
- ZDB-FIG-180320-1
- Publication
- Gurkov et al., 2017 - Microencapsulated fluorescent pH probe as implantable sensor for monitoring the physiological state of fish embryos
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Description of pH-sensitive MBMs used. (a) General scheme of preparation of MBMs using the LbL method: co-precipitation of SNARF-1-D (purple) into porous CaCO3 cores (yellow); LbL assembly of microcapsule shell around the cores (only three layers of negatively charged polymer, three layers of positively charged polymer and final biocompatible layer are depicted); and dissolution of cores. (b) SEM image of porous CaCO3 cores with incorporated SNARF-1-D. (c) Prepared MBMs under confocal laser scanning microscope LSM 700. (d) Examples of fluorescence spectra of microencapsulated SNARF-1-D with varying pH. (e) Calibration curve of MBMs containing SNARF-1-D with varying pH was built based on median values (emphasized by larger dark blue points); original values are also depicted (smaller light blue points). |