Fig. 1

Fig. 1

(a) General scheme of preparation of MBMs using the LbL method: co-precipitation of SNARF-1-D (purple) into porous CaCO₃ cores (yellow); LbL assembly of microcapsule shell around the cores (only three layers of negatively charged polymer, three layers of positively charged polymer and final biocompatible layer are depicted); and dissolution of cores. (b) SEM image of porous CaCO₃ cores with incorporated SNARF-1-D. (c) Prepared MBMs under confocal laser scanning microscope LSM 700. (d) Examples of fluorescence spectra of microencapsulated SNARF-1-D with varying pH. (e) Calibration curve of MBMs containing SNARF-1-D with varying pH was built based on median values (emphasized by larger dark blue points); original values are also depicted (smaller light blue points).