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ZFIN ID: ZDB-FIG-180202-34
Jinchao et al., 2017 - Thrombopoietic stimulating activity of rhTyrRS (Y341A). Scientific Reports   7:12184 Full text @ Sci. Rep.
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec

Fig. 4

The effect of rhTyrRS (Y341A) on zebrafish thromboblasts. Green areas represent the thromboblasts labeled by GFP in zebrafish. The changes in thromboblast numbers in zebrafish at 24 h after micro-injection of rhTyrRS (Y341A) (0.002, 0.02, or 0.2 ng per fish) are shown in (A,B,C). (J) Statistics on the number of thromboblasts in zebrafish at different time points (P < 0.001); the abscissa indicates the observation time after microinjection. The ordinate indicates the number of thromboblasts. The changes in thromboblast counts in the PBS, rhTyrRS (Y341A) (2 × 10−2 ng per fish) group, and TPO mRNA (0.05 ng per fish) group at 24 h after micro-injection are shown in (D,E,F). (K) Statistics on the number of thromboblasts in zebrafish at different time points (P < 0.001). The abscissa indicates the observation time after microinjection. The ordinate indicates the number of thromboblasts. Laser confocal microscope observation of thromboblast adhesion to vascular endothelial cells 24 h after micro-injection. (G) PBS group. (H) rhTyrRS (Y341A) (0.02 ng per fish) group. (I) TPO mRNA (0.05 ng per fish) group. (L) Statistics on the number of thromboblasts adhering to vascular endothelial cells in caudal hematopoietic tissue. The abscissa indicates the observation time after micro-injection; the ordinate indicates the number of thromboblast adhesion (***P < 0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP la2Tg; zf2020Tg control Long-pec thromboblast IFL
mCherry la2Tg; zf2020Tg control Long-pec vascular endothelium IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.