Fig. S3
Rac1 inhibition specifically impairs endoderm internalization. Control endodermal cells were transplanted just beneath the EVL (A), and dnRac1-expressing endodermal cells were transplanted just beneath the EVL (B) or close to the YSL (C). At 24 hpf, while control cells contribute to endodermal derivatives (A), endodermal cells expressing dnRac1 transplanted just beneath the EVL are found in ectodermal derivatives (B). (C) Importantly, dnRac1-expressing cells transplanted close to the YSL contribute to endodermal derivatives at 24 hpf. (D) Distribution of embryos with transplanted cells in endodermal derivatives at 24 hpf. ***P < 0.001. (Scale bar: 100 μm.) |