Fig. 2

Nerve regrowth at the fin base requires sox10 and FGF.

(A-C) Quantification of fin nerve regrowth in Tg(mnx1:GFP) transgenic larvae 24 hours after fin removal; nerves show different degrees of nerve regrowth (A-C). Categories 0, 1, and 2 indicate that none (A, A'), one (B, B'), or both sides (C,C') of the ring-like nerve network at the fin base regrew, respectively. (A'-C'): schematic representation of these results. (D-F) Nerve regrowth at the fin base 24 hours after fin removal in a wild type sibling (D) and a sox10 mutant (E). Sox10 mutants display significantly reduced nerve regrowth compared to wild type siblings. Graphical representation of the extent of nerve regrowth (category 0, 1, 2; F). (G-J) Nerve regrowth at the fin base at 24 hours after fin removal in a control larva treated with DMSO (G) and larvae treated with a low (17μM, H) or a high dose (34μM, I) of the FGF inhibitor SU5402. DMSO or SU5402 were added immediately after fin amputation. FGF inhibitor-treated larvae show significantly less nerve regrowth compared to DMSO-treated controls. Graphical representation of the extent of nerve regrowth (category 0, 1, 2; J). All scale bars are 10 μm.