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ZFIN ID: ZDB-FIG-171229-21
Tarifeño-Saldivia et al., 2017 - Transcriptome analysis of pancreatic cells across distant species highlights novel important regulator genes. BMC Biology   15:21 Full text @ BMC Biol.
Anatomical Terms:
Stage Range: Protruding-mouth to Adult

Fig. S1 Expression of the transgenes Tg(ins:GFP), Tg(gcga:GFP), Tg(sst2:GFP), and Tg(ins:NTR:mCherry) in the endocrine pancreatic cell types. (A–C) Whole mount immunostaining of 3 dpf transgenic larvae Tg(ins:GFP) demonstrating the selective expression of GFP in beta cells. (D–I) Immunostaining of pancreas sections from adult transgenic fish Tg(gcga:GFP/ins:NTR:mCherry). Glucagon+ cells (see arrows in D–F) express high level of GFP and are not labelled by mCherry, while many insulin expressing cells are labelled by mCherry and by GFP (at slightly lower levels) (see arrowheads in G–I). These data reveal a leaking expression of the gcga:GFP transgene in beta cells. (J–L) ISH performed on pancreas section of adult Tg(sst2:gfp) with sst2 probe followed by immunofluorescence using the GFP antibody; the expression of endogenous sst2 gene co-localize with GFP staining confirming the specific expression of the transgene in delta cells [28].

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ulg021Tg standard conditions Protruding-mouth pancreatic B cell IHC
GFP gz18Tg standard conditions Adult pancreatic D cell IHC
jh4Tg ; ia1Tg standard conditions Adult pancreatic A cell IHC
Adult pancreatic B cell IHC
mCherry jh4Tg ; ia1Tg standard conditions Adult pancreatic B cell IHC
sst2 gz18Tg standard conditions Adult pancreatic D cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.