ZFIN ID: ZDB-FIG-171228-20
Lawir et al., 2017 - A missense mutation in zbtb17 blocks the earliest steps of T cell differentiation in zebrafish. Scientific Reports   7:44145 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Pec-fin

Fig. 4

Unperturbed haematopoietic development in zbtb17 mutants.

(A) Microphotographs of wild-type and zbtb17 mutant embryos after RNA in situ hybridization with the indicated gene-specific probes and at the indicated times of development (scl, 36 hpf; myb, 36 hpf; gata1, 24 hpf). Scale bar, 100 µm (B) Representative microphotographs of control and morphant embryos on the ikaros:eGFP transgenic background to visualize lymphoid progenitors in the thymus and the caudal haematopoietic tissue (CHT) at 63 hpf. (C,D) Thymus homing assay in control and morphant embryos. Summary statistics of cohorts of embryos (examples in (B)) for cell counts at 63 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fr101Tg control Pec-fin caudal hematopoietic tissue IFL
Pec-fin thymus primordium lymphoid progenitor cell IFL
fr101Tg + MO1-socs3b standard conditions Pec-fin caudal hematopoietic tissue IFL
Pec-fin thymus primordium lymphoid progenitor cell IFL
fr101Tg + MO1-zbtb17 standard conditions Pec-fin caudal hematopoietic tissue IFL
Pec-fin thymus primordium lymphoid progenitor cell IFL
fr101Tg + MO3-socs1a standard conditions Pec-fin caudal hematopoietic tissue IFL
Pec-fin thymus primordium lymphoid progenitor cell IFL
gata1a WT standard conditions Prim-5 erythroid lineage cell ISH
zbtb17t20463/t20463 standard conditions Prim-5 erythroid lineage cell ISH
myb WT standard conditions Prim-25 hematopoietic stem cell ISH
zbtb17t20463/t20463 standard conditions Prim-25 hematopoietic stem cell ISH
tal1 WT standard conditions Prim-25 hematopoietic stem cell ISH
zbtb17t20463/t20463 standard conditions Prim-25 hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fr101Tg + MO1-zbtb17 standard conditions Pec-fin T cell migration decreased occurrence, abnormal
Pec-fin thymus lymphoid progenitor cell decreased amount, abnormal
fr101Tg + MO3-socs1a standard conditions Pec-fin T cell migration decreased occurrence, abnormal
Pec-fin thymus lymphoid progenitor cell decreased amount, abnormal
zbtb17t20463/t20463 standard conditions Prim-5 erythroid lineage cell gata1a expression increased amount, abnormal
Prim-25 hematopoietic stem cell myb expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.