FIGURE

Fig. S1

ID

ZDB-FIG-171101-45

Publication

Yue et al., 2015 - A co-culture assay of embryonic zebrafish hearts to assess migration of epicardial cells in vitro

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Fig. S1

Hearts extracted at 60 hpf lack epicardial cells. (A and B) Confocal micrographs of cmlc2:EGFP; tcf21:DsRed2 hearts extracted at 60 hpf . Images show brightest point projections from confocal z-series. (A) cmlc2:EGFP; tcf21:DsRed2 hearts before being placed into culture (Day 0). (B), cmlc2:EGFP; tcf21:DsRed2 hearts after 7 days in culture (Day 7). There were no epicardial cells (red) observed on the heart myocardia (green) at Day 0 or Day 7. In addition, there were no observed tcf21- cells with the stereotypical flattened phenotype of epicardial cells present on top of the myocardium (blue, DAPI nuclear staining). Scale bars in all images represent 50 μm.

Expression Data

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.