ZFIN ID: ZDB-FIG-171020-7
Vincent et al., 2017 - Neutrophil derived LTB4 induces macrophage aggregation in response to encapsulated Streptococcus iniae infection. PLoS One   12:e0179574 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 4

Neutrophil crosstalk drives macrophage aggregate formation.

(A) Double transgenic Tg(mpeg:dendra2) x Tg(mpx:mCherry) larvae show that neutrophils are present in and around macrophage aggregates. Images at 40X; scale bar is 20 μm. (B) Representative 20X images of Rac2WT or Rac2D57N larvae at 24 hpi following inoculation. Tg(mpx:mCherry-2a-rac2wt) (Rac2WT) or Tg(mpx:mCherry-2a-rac2d57n) (Rac2D57N) were crossed to Tg(mpeg1:dendra2) and the resulting double transgenic larvae were infected with 50 CFU WT S. iniae or mock-infected with PBS. Rac2D57N larvae are defective for aggregate formation. Scale bar is 80 μm. (C) Quantification of the average total percent of larvae forming macrophage aggregates from (B).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Dendra2 uwm12Tg ; zf306Tg bacterial treatment by injection: Streptococcus iniae Protruding-mouth to Day 4 macrophage IFL
zf307Tg ; uwm12Tg bacterial treatment by injection: Streptococcus iniae Protruding-mouth to Day 4 macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
uwm12Tg ; zf306Tg bacterial treatment by injection: Streptococcus iniae Protruding-mouth - Day 4 macrophage aggregated, abnormal
zf307Tg ; uwm12Tg bacterial treatment by injection: Streptococcus iniae Protruding-mouth - Day 4 macrophage aggregated, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One