FIGURE

Fig. 4

ID

ZDB-FIG-171020-7

Publication

Vincent et al., 2017 - Neutrophil derived LTB4 induces macrophage aggregation in response to encapsulated Streptococcus iniae infection

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Fig. 4

Neutrophil crosstalk drives macrophage aggregate formation.

(A) Double transgenic Tg(mpeg:dendra2) x Tg(mpx:mCherry) larvae show that neutrophils are present in and around macrophage aggregates. Images at 40X; scale bar is 20 μm. (B) Representative 20X images of Rac2WT or Rac2D57N larvae at 24 hpi following inoculation. Tg(mpx:mCherry-2a-rac2wt) (Rac2WT) or Tg(mpx:mCherry-2a-rac2d57n) (Rac2D57N) were crossed to Tg(mpeg1:dendra2) and the resulting double transgenic larvae were infected with 50 CFU WT S. iniae or mock-infected with PBS. Rac2D57N larvae are defective for aggregate formation. Scale bar is 80 μm. (C) Quantification of the average total percent of larvae forming macrophage aggregates from (B).

Expression Data

Gene:	Dendra2
Fish:	uwm12Tg; zf306Tg uwm12Tg; zf307Tg
Condition:	bacterial treatment by injection: Streptococcus iniae
Anatomical Term:	macrophage
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	uwm12Tg; zf306Tg uwm12Tg; zf307Tg
Condition:	bacterial treatment by injection: Streptococcus iniae
Observed In:	macrophage
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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