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Figures for Vincent et al., 2017


Figure Caption/Comments:

Fig. 4

Neutrophil crosstalk drives macrophage aggregate formation.

(A) Double transgenic Tg(mpeg:dendra2) x Tg(mpx:mCherry) larvae show that neutrophils are present in and around macrophage aggregates. Images at 40X; scale bar is 20 μm. (B) Representative 20X images of Rac2WT or Rac2D57N larvae at 24 hpi following inoculation. Tg(mpx:mCherry-2a-rac2wt) (Rac2WT) or Tg(mpx:mCherry-2a-rac2d57n) (Rac2D57N) were crossed to Tg(mpeg1:dendra2) and the resulting double transgenic larvae were infected with 50 CFU WT S. iniae or mock-infected with PBS. Rac2D57N larvae are defective for aggregate formation. Scale bar is 80 μm. (C) Quantification of the average total percent of larvae forming macrophage aggregates from (B).

Figure Data:
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