| ZFIN ID: ZDB-FIG-171011-10 |
Joo et al., 2013
- CCDC41 is required for ciliary vesicle docking to the mother centriole..
Proceedings of the National Academy of Sciences of the United States of America
110(15):5987-92
Full text @ Proc. Natl. Acad. Sci. USA
EXPRESSION / LABELING:
| Gene: | |
|---|---|
| Fish: | |
| Anatomical Terms: | |
| Stage: | Prim-5 |
PHENOTYPE:
| Fish: | |
|---|---|
| Knockdown Reagent: | |
| Observed In: | |
| Stage: | Day 5 |
Fig. 3 ccdc41 is expressed in ciliated tissues and its depletion induces olfactory ciliogenesis defects in zebrafish. (A) Alignment of human and zebrafish CCDC41 protein sequences by using the ClustalX program. (B) ccdc41 whole-mount in situ hybridization shows that ccdc41 mRNA is expressed in olfactory placodes (arrow), pronephric duct (arrowhead), and tail myotomes at 24 h postfertilization. (Inset) Frontal view of the head shows ccdc41 expression in bilateral olfactory placodes. (C) Anti–acetylated-tubulin (Act-Tub) antibody staining of the zebrafish nasal pit at 5 d postfertilization. MO, morpholino. |
Gene Expression Details
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| cep83 | WT | standard conditions | Prim-5 | olfactory placode | ISH | |
| Prim-5 | post-vent region myotome | ISH | ||||
| Prim-5 | pronephric duct | ISH |
Antibody Labeling Details
No data available
Phenotype Details
| Fish | Conditions | Stage | Phenotype |
|---|---|---|---|
| WT + MO1-cep83 | standard conditions | Day 5 | cilium assembly disrupted, abnormal |
| Day 5 | olfactory pit cilium decreased amount, abnormal |
| Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA |
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