FIGURE

Fig. 4 S1

ID

ZDB-FIG-171004-6

Publication

Boothe et al., 2017 - A tunable refractive index matching medium for live imaging cells, tissues and model organisms

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4 S1

Improved nuclear segmentation in deep tissue layers by Iodixanol supplementation.

four hpf zebrafish embryos expressing RFP-PCNA. Top row: Maximum projection (left) and optical XY-section at 200 �m depth (right) of an embryo in regular media (RI = 1.333) highlights a progressive loss of nuclear signal detection and segmentation beyond 100 �m imaging depth, which is quantified in the scatter plot of mean nuclear intensity versus depth (right). Bottom row: RI tuning with Iodixanol to RI = 1.363 enables nuclear detection and segmentation at up to 300 �m imaging depth. Panel order and imaging conditions exactly as above. Scale bars = 50 �m. The colour scheme encodes relative intensity (brightest = white).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife