PUBLICATION

A tunable refractive index matching medium for live imaging cells, tissues and model organisms

Authors
Boothe, T., Hilbert, L., Heide, M., Berninger, L., Huttner, W.B., Zaburdaev, V., Vastenhouw, N.L., Myers, E.W., Drechsel, D.N., Rink, J.C.
ID
ZDB-PUB-170715-4
Date
2017
Source
eLIFE   6: (Journal)
Registered Authors
Vastenhouw, Nadine
Keywords
cell biology, developmental biology, stem cells, zebrafish
MeSH Terms
  • Solutions
  • Humans
  • Embryo, Nonmammalian/physiology
  • Refractometry/methods*
  • Solvents
  • Zebrafish/embryology
  • Models, Biological
  • Animals
  • HeLa Cells
  • Cell Survival
  • Imaging, Three-Dimensional*
  • Triiodobenzoic Acids/pharmacology*
  • Tissue Culture Techniques
(all 13)
PubMed
28708059 Full text @ Elife
Abstract
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus a substantial improvement of the live-imaging of primary cell cultures, planarians, zebrafish and human cerebral organoids.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cbg7TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    No data available
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    RFPEFGRFP
    1 - 1 of 1
    Show
    Mapping
    No data available
    Your Input Welcome
    We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
    Citation
    Boothe, T., Hilbert, L., Heide, M., Berninger, L., Huttner, W.B., Zaburdaev, V., Vastenhouw, N.L., Myers, E.W., Drechsel, D.N., Rink, J.C. (2017) A tunable refractive index matching medium for live imaging cells, tissues and model organisms. eLIFE. 6.