ZFIN ID: ZDB-FIG-171002-5
Shimizu et al., 2017 - The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 5

Blocking MuRF1-mediated proteasome degradation preserves myofibril integrity in tre/ncx1 deficient hearts.

(A) Z-lines were visualized by α-actinin staining. By 72 hpf, sarcomeres are disassembled in hearts of uninjected (tre) and control morpholino-injected (tre +ctlMO) tremblor embryos. Murf1a/murf1 b knockdown does not affect sarcomere integrity in wild type embryos (WT +MO), but prevents sarcomere degradation in tre (tre +MO). Similarly, treatment with a proteasome inhibitor, MG132, preserves myofibril integrity in tre cardiomyocytes (tre +MG132). Scale bar, 10 μm. (B) Graph shows % of embryos with periodic α-actinin staining at 72 hpf. (C) Western blot detecting MuRF1 and β-actin proteins in uninjected control (WT control) and murf1a/murf1 b knockdown (MuRF1 MO) embryos. Chi-squared test, *p<0.05; **p<0.01; ***p<0.001.
Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-trim63 WB WT control Protruding-mouth whole organism
WB WT + MO1-trim63a + MO1-trim63b standard conditions Protruding-mouth whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-trim63a + MO1-trim63b standard conditions Protruding-mouth cardiac muscle cell sarcomere structure, normal
Protruding-mouth whole organism Ab1-trim63 labeling decreased amount, abnormal
slc8a1atc318d/tc318d standard conditions Protruding-mouth cardiac muscle cell sarcomere disassembled, abnormal
Protruding-mouth cardiac muscle cell Z disc decreased amount, abnormal
slc8a1atc318d/tc318d chemical treatment: proteasome inhibitor Protruding-mouth cardiac muscle cell sarcomere structure, ameliorated
Protruding-mouth cardiac muscle cell Z disc amount, ameliorated
slc8a1atc318d/tc318d + MO1-trim63a + MO1-trim63b standard conditions Protruding-mouth cardiac muscle cell sarcomere structure, ameliorated
Protruding-mouth cardiac muscle cell Z disc amount, ameliorated
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife
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