ZFIN ID: ZDB-FIG-171002-5
Shimizu et al., 2017 - The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 5

Blocking MuRF1-mediated proteasome degradation preserves myofibril integrity in tre/ncx1 deficient hearts.

(A) Z-lines were visualized by α-actinin staining. By 72 hpf, sarcomeres are disassembled in hearts of uninjected (tre) and control morpholino-injected (tre +ctlMO) tremblor embryos. Murf1a/murf1 b knockdown does not affect sarcomere integrity in wild type embryos (WT +MO), but prevents sarcomere degradation in tre (tre +MO). Similarly, treatment with a proteasome inhibitor, MG132, preserves myofibril integrity in tre cardiomyocytes (tre +MG132). Scale bar, 10 μm. (B) Graph shows % of embryos with periodic α-actinin staining at 72 hpf. (C) Western blot detecting MuRF1 and β-actin proteins in uninjected control (WT control) and murf1a/murf1 b knockdown (MuRF1 MO) embryos. Chi-squared test, *p<0.05; **p<0.01; ***p<0.001.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-trim63 WB WT control Protruding-mouth whole organism
WB WT + MO1-trim63a + MO1-trim63b standard conditions Protruding-mouth whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-trim63a + MO1-trim63b standard conditions Protruding-mouth cardiac muscle cell sarcomere structure, normal
Protruding-mouth whole organism Ab1-trim63 labeling decreased amount, abnormal
slc8a1atc318d/tc318d standard conditions Protruding-mouth cardiac muscle cell sarcomere disassembled, abnormal
Protruding-mouth cardiac muscle cell Z disc decreased amount, abnormal
slc8a1atc318d/tc318d chemical treatment: proteasome inhibitor Protruding-mouth cardiac muscle cell sarcomere structure, ameliorated
Protruding-mouth cardiac muscle cell Z disc amount, ameliorated
slc8a1atc318d/tc318d + MO1-trim63a + MO1-trim63b standard conditions Protruding-mouth cardiac muscle cell sarcomere structure, ameliorated
Protruding-mouth cardiac muscle cell Z disc amount, ameliorated
Acknowledgments:
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