ZFIN ID: ZDB-FIG-171002-5 |
Antibody: | |
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Fish: | |
Knockdown Reagents: | |
Anatomical Term: | |
Stage: | Protruding-mouth |
Fish: | |
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Condition: | |
Knockdown Reagents: | |
Observed In: | |
Stage: | Protruding-mouth |
Fig. 5 Blocking MuRF1-mediated proteasome degradation preserves myofibril integrity in tre/ncx1 deficient hearts. (A) Z-lines were visualized by α-actinin staining. By 72 hpf, sarcomeres are disassembled in hearts of uninjected (tre) and control morpholino-injected (tre +ctlMO) tremblor embryos. Murf1a/murf1 b knockdown does not affect sarcomere integrity in wild type embryos (WT +MO), but prevents sarcomere degradation in tre (tre +MO). Similarly, treatment with a proteasome inhibitor, MG132, preserves myofibril integrity in tre cardiomyocytes (tre +MG132). Scale bar, 10 μm. (B) Graph shows % of embryos with periodic α-actinin staining at 72 hpf. (C) Western blot detecting MuRF1 and β-actin proteins in uninjected control (WT control) and murf1a/murf1 b knockdown (MuRF1 MO) embryos. Chi-squared test, *p<0.05; **p<0.01; ***p<0.001. |
Antibody | Assay | Fish | Conditions | Stage | Anatomy |
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Ab1-trim63 | WB | WT | control | Protruding-mouth | whole organism |
WB | WT + MO1-trim63a + MO1-trim63b | standard conditions | Protruding-mouth | whole organism |
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