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ZIRC
ZFIN ID: ZDB-FIG-171002-1
Shimizu et al., 2017 - The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. 1

Disorganized myofibril structure in tre/ncx1h cardiomyocytes.

Wild type (WT) and tre/ncx1h (tre) mutant hearts at 30, 48 and 72 hpf. (A) Zebrafish hearts stained for α-actinin to visualize Z-lines. At 30 hpf, periodic α-actitin staining was observed in wild type and tre hearts (arrowheads). By 48 hpf, sarcomeres are disassembled in tre hearts. Scale bar, 10 μm. (B) The cell shape of cardiomyocytes was visualized by Zn8 staining. Scale bar, 10 μm. (C) Embryonic fish hearts were visualized by GFP expression in the myl7:EGFP transgenic background. Note that tre hearts become dysmorphic after two days of development. Scale bar, 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP slc8a1atc318d/tc318d; twu34Tg standard conditions Prim-15 heart tube IFL
Long-pec heart IFL
Protruding-mouth heart IFL
twu34Tg standard conditions Prim-15 heart tube IFL
Long-pec heart IFL
Protruding-mouth heart IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-8 IHC WT standard conditions Prim-15 cardiac muscle cell
IHC Long-pec cardiac muscle cell
IHC Protruding-mouth cardiac muscle cell
IHC slc8a1atc318d/tc318d standard conditions Prim-15 cardiac muscle cell
IHC Long-pec cardiac muscle cell
IHC Protruding-mouth cardiac muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
slc8a1atc318d/tc318d standard conditions Prim-15 cardiac muscle cell sarcomere structure, normal
Long-pec cardiac muscle cell decreased length, abnormal
Long-pec cardiac muscle cell shape, abnormal
Long-pec cardiac muscle cell cardiac myofibril disorganized, abnormal
Long-pec cardiac muscle cell sarcomere disassembled, abnormal
Long-pec cardiac muscle cell sarcomere disorganized, abnormal
Protruding-mouth cardiac muscle cell decreased length, abnormal
Protruding-mouth cardiac muscle cell shape, abnormal
Protruding-mouth cardiac muscle cell cardiac myofibril disorganized, abnormal
Protruding-mouth cardiac muscle cell sarcomere disassembled, abnormal
Protruding-mouth cardiac muscle cell sarcomere disorganized, abnormal
slc8a1atc318d/tc318d; twu34Tg standard conditions Long-pec atrium morphology, abnormal
Long-pec cardiac ventricle morphology, abnormal
Protruding-mouth atrium morphology, abnormal
Protruding-mouth cardiac ventricle morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife