FIGURE

Fig. 5

ID

ZDB-FIG-170926-6

Publication

Paatero et al., 2017 - Analyses in zebrafish embryos reveal that nanotoxicity profiles are dependent on surface-functionalization controlled penetrance of biological membranes

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Surface functionalization affects aggregation of MSNs on the embryo skin. The uptake of the nanoparticles from the aquaeous medium into the embryo was analyzed by mounting anesthesized living embryos into agarose and imaged using confocal microscopy (Leica SP5 Matrix). A transgenic strain (kdrl:EGFP) expressing a GFP labeled vasculature were used 24hpf embryos were incubated 48 h with the highest tolerated dose of the different nanoparticles e.g 100 μg/ml NH2-, SUCC- and PEG-MSNs, and 10 ug/ml of PEI-MSNs. DMSO treated embryos were used as controls and show background fluoresence (A). Maximum Z-projections are shown. The white arrows mark nanoparticle clusters on the superficial layer of the embryo.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.