Fig. 6
- ID
- ZDB-FIG-170912-9
- Publication
- Xie et al., 2016 - S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence
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In Gα13-deficient embryos, the endocardial-cell architecture is disrupted. Whole-mount ZO-1 immunostaining was performed in Tg(kdrl: moesin-GFP) embryos at 15 s (A, B) Projections of confocal Z-stacks of endocardial cells showing ZO-1 expression (magenta) in endocardial precursors (green). (A1-A2′, B-B2′) Single confocal Z-plane images of areas shown in boxes in A, B. (A1, B1) A Z-plane image in the ventral region showing the ZO-1 expression. (A1′, B1′) Images of ZO-1 expression only in A1, B1. (A2, B2) A Z-plane image 3 µm dorsal to that in A1, B1. (A2′, B2′) Images of ZO-1 expression only in A2, B2. (A3, A3′, B3, B3′) Images of XZ transverse sections of the regions indicated by white dashed lines in A1, B1. (C) Ratio of ZO-1 intensity in the dorsal/ventral region of endocardial cells. 5 embryos in each group. P<0.001. Yellow dashed line: midline; white and yellow arrowheads: ZO-1 expression in control and gna13a/b MO-injected embryos, respectively; D: dorsal; V: ventral. |
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| Fish: | |
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| Anatomical Term: | |
| Stage: | 14-19 somites |
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| Observed In: | |
| Stage: | 14-19 somites |
Reprinted from Developmental Biology, 414, Xie, H., Ye, D., Sepich, D., Lin, F., S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence, 228-43, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.