|ZFIN ID: ZDB-FIG-170822-19|
Alterations in Notch signaling mediate podocyte and interrenal genesis. (A,B) Gain and loss of function Notch experiments with Tg(hsp70::GAL4, UAS::NICD) and DAPT chemical treatments were performed in WT and brca2 morphants to assess podocyte development by WISH for wt1b and interrenal development by WISH for nr5a1a. Embryos are shown in dorsal views, where black boxes demarcate the cervical region where the podocytes and interrenal gland develop. (C,D) The standard deviation of triplicate groups, consisting of at least 5 embryos per group, was statistically analyzed by ANOVA with Tukey test. WT embryos had statistically significant reduced podocytes with DAPT. brca2 morphants failed to develop podocytes after NICD or DAPT. brca2 morphants had a reduced interrenal field following NICD that was statistically significant compared to non-heat shocked controls. Asterisks (*) over black bars indicate p < 0.05 between the respective groups, and the black bar in (C) indicates that the brca2 MO1 groups are not significantly different from each other while all are significantly different from the WT Control, NICD and DAPT groups.
|nr5a1a||WT||chemical treatment by environment: DAPT||Prim-5||interrenal primordium||ISH|
|WT||heat shock||Prim-5||interrenal primordium||ISH|
|WT + MO2-brca2||chemical treatment by environment: DAPT||Prim-5||interrenal primordium||ISH|
|WT + MO2-brca2||heat shock||Prim-5||interrenal primordium||ISH|
|kca3Tg; kca4Tg||heat shock||Prim-5||interrenal primordium||ISH|
|kca3Tg; kca4Tg + MO2-brca2||heat shock||Prim-5||interrenal primordium||ISH|
|wt1b||WT||chemical treatment by environment: DAPT||Prim-5||pronephric podocyte||ISH|
|WT||heat shock||Prim-5||pronephric podocyte||ISH|
|WT + MO2-brca2||chemical treatment by environment: DAPT||Prim-5||Not Detected||pronephric podocyte||ISH|
|WT + MO2-brca2||heat shock||Prim-5||Not Detected||pronephric podocyte||ISH|
|kca3Tg; kca4Tg||heat shock||Prim-5||pronephric podocyte||ISH|
|kca3Tg; kca4Tg + MO2-brca2||heat shock||Prim-5||Not Detected||pronephric podocyte||ISH|
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Developmental Biology, 428(1), Kroeger, P.T., Drummond, B.E., Miceli, R., McKernan, M., Gerlach, G.F., Marra, A.N., Fox, A., McCampbell, K.K., Leshchiner, I., Rodriguez-Mari, A., BreMiller, R., Thummel, R., Davidson, A.J., Postlethwait, J., Goessling, W., Wingert, R.A., The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development, 148-163, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.