ZFIN ID: ZDB-FIG-170822-19
Kroeger et al., 2017 - The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development. Developmental Biology   428(1):148-163 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 11

Alterations in Notch signaling mediate podocyte and interrenal genesis. (A,B) Gain and loss of function Notch experiments with Tg(hsp70::GAL4, UAS::NICD) and DAPT chemical treatments were performed in WT and brca2 morphants to assess podocyte development by WISH for wt1b and interrenal development by WISH for nr5a1a. Embryos are shown in dorsal views, where black boxes demarcate the cervical region where the podocytes and interrenal gland develop. (C,D) The standard deviation of triplicate groups, consisting of at least 5 embryos per group, was statistically analyzed by ANOVA with Tukey test. WT embryos had statistically significant reduced podocytes with DAPT. brca2 morphants failed to develop podocytes after NICD or DAPT. brca2 morphants had a reduced interrenal field following NICD that was statistically significant compared to non-heat shocked controls. Asterisks (*) over black bars indicate p < 0.05 between the respective groups, and the black bar in (C) indicates that the brca2 MO1 groups are not significantly different from each other while all are significantly different from the WT Control, NICD and DAPT groups.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nr5a1a WT chemical treatment by environment: DAPT Prim-5 interrenal primordium ISH
WT heat shock Prim-5 interrenal primordium ISH
WT + MO2-brca2 chemical treatment by environment: DAPT Prim-5 interrenal primordium ISH
WT + MO2-brca2 heat shock Prim-5 interrenal primordium ISH
kca3Tg; kca4Tg heat shock Prim-5 interrenal primordium ISH
kca3Tg; kca4Tg + MO2-brca2 heat shock Prim-5 interrenal primordium ISH
wt1b WT chemical treatment by environment: DAPT Prim-5 pronephric podocyte ISH
WT heat shock Prim-5 pronephric podocyte ISH
WT + MO2-brca2 chemical treatment by environment: DAPT Prim-5 Not Detected pronephric podocyte ISH
WT + MO2-brca2 heat shock Prim-5 Not Detected pronephric podocyte ISH
kca3Tg; kca4Tg heat shock Prim-5 pronephric podocyte ISH
kca3Tg; kca4Tg + MO2-brca2 heat shock Prim-5 Not Detected pronephric podocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: DAPT Prim-5 pronephric glomerulus has fewer parts of type pronephric podocyte, abnormal
Prim-5 pronephric podocyte wt1b expression decreased distribution, abnormal
WT heat shock Prim-5 pronephric glomerulus has normal numbers of parts of type pronephric podocyte, normal
WT + MO2-brca2 chemical treatment by environment: DAPT Prim-5 interrenal primordium nr5a1a expression increased distribution, abnormal
Prim-5 pronephric podocyte wt1b expression absent, abnormal
Prim-5 pronephros lacks parts or has fewer parts of type pronephric podocyte, abnormal
WT + MO2-brca2 heat shock Prim-5 interrenal primordium nr5a1a expression increased distribution, abnormal
Prim-5 pronephric podocyte wt1b expression absent, abnormal
Prim-5 pronephros lacks parts or has fewer parts of type pronephric podocyte, abnormal
kca3Tg; kca4Tg + MO2-brca2 heat shock Prim-5 interrenal primordium nr5a1a expression spatial pattern, ameliorated
Prim-5 pronephric podocyte wt1b expression absent, abnormal
Prim-5 pronephros lacks parts or has fewer parts of type pronephric podocyte, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 428(1), Kroeger, P.T., Drummond, B.E., Miceli, R., McKernan, M., Gerlach, G.F., Marra, A.N., Fox, A., McCampbell, K.K., Leshchiner, I., Rodriguez-Mari, A., BreMiller, R., Thummel, R., Davidson, A.J., Postlethwait, J., Goessling, W., Wingert, R.A., The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development, 148-163, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.