ZFIN ID: ZDB-FIG-170816-14
Alghanem et al., 2017 - RCAN1.4 regulates VEGFR-2 internalisation, cell polarity and migration in human microvascular endothelial cells. Angiogenesis   20(3):341-358 Full text @ Angiogenesis
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 9

RCAN1.4 regulates endothelial cell sprouting and vascular formation in zebrafish. a In vitro sprouting angiogenesis assay. HDMECs were transfected with control non-targeting siRNA (N.S. siRNA) or RCAN1 siRNA prior to incubation with cell tracker dye green or orange. Cells were mixed 1:1 and incubated with carboxymethycellulose in ULA 96-well plates for 18 h prior to embedding in collagen matrix. Cells were stimulated with VEGF (50 ng/ml) for 18 h and fixed in 2% PFA prior to fluorescence imaging. b Quantification of endothelial tip and stalk cell formation. Sprouting tubes from VEGF treated spheroids were quantified (mean ± S.D., n = 3). ***P < 0.001 (unpaired Student’s t test). c Transgenic fli1-GFP zebrafish embryos were injected with mismatch or RCAN1a-4 morpholino oligonucleotides and imaged at 48 hpf. Scale bar represents 500 μm. d Analyses of total vessel length and junction number, n = 14 for mismatch, n = 15 for RCAN morpholino. Asterisk indicates P < 0.01 (unpaired student’s t test)

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-rcan1a standard conditions Long-pec dorsal longitudinal anastomotic vessel angiogenic sprout fusion arrested, abnormal
Long-pec intersegmental vessel decreased branchiness, abnormal
Long-pec sprouting angiogenesis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Angiogenesis for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Angiogenesis