IMAGE

Fig. 9

ID
ZDB-IMAGE-170816-14
Source
Image
Figure Caption

Fig. 9

RCAN1.4 regulates endothelial cell sprouting and vascular formation in zebrafish. a In vitro sprouting angiogenesis assay. HDMECs were transfected with control non-targeting siRNA (N.S. siRNA) or RCAN1 siRNA prior to incubation with cell tracker dye green or orange. Cells were mixed 1:1 and incubated with carboxymethycellulose in ULA 96-well plates for 18 h prior to embedding in collagen matrix. Cells were stimulated with VEGF (50 ng/ml) for 18 h and fixed in 2% PFA prior to fluorescence imaging. b Quantification of endothelial tip and stalk cell formation. Sprouting tubes from VEGF treated spheroids were quantified (mean ± S.D., n = 3). ***P < 0.001 (unpaired Student’s t test). c Transgenic fli1-GFP zebrafish embryos were injected with mismatch or RCAN1a-4 morpholino oligonucleotides and imaged at 48 hpf. Scale bar represents 500 μm. d Analyses of total vessel length and junction number, n = 14 for mismatch, n = 15 for RCAN morpholino. Asterisk indicates P < 0.01 (unpaired student’s t test)

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Angiogenesis