ZFIN ID: ZDB-FIG-170815-8
Garcia et al., 2017 - Sheath Cell Invasion and Trans-differentiation Repair Mechanical Damage Caused by Loss of Caveolae in the Zebrafish Notochord. Current biology : CB   27(13):1982-1989.e3 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

Release of Vacuolated Cell Contents Triggers Sheath Cell Invasion

(A and B) Confocal images of live WT embryos expressing sag:gal4::UAS:mcherry-NTR in vacuolated cells and col9a2:GFPCaaX in sheath cells.

(C and D) In embryos treated with 1.5 mM metronidazole (MTZ), sheath cells invade areas of vacuolated cell death. Arrows point to invading sheath cells; asterisks mark intact vacuolated cells.

(E) LC-MS quantitation of ATP and UTP levels in nucleus pulposus (NP) and annulus fibrosis (AF) tissue isolated from 6-month-old pig spines. n = 12 for NP and 4 for AF. ∗∗∗p < 0.001, Student’s t test.

The scale bars represent 100 μm. Error bars are SD. See also Figures S3 and S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP c264Tg; nksagff214aGt; pd1151Tg (EKW) standard conditions Long-pec notochord outer sheath cell IFL
Protruding-mouth notochord outer sheath cell IFL
c264Tg; nksagff214aGt; pd1151Tg (EKW) chemical treatment by environment: metronidazole Long-pec notochord outer sheath cell IFL
Protruding-mouth notochord outer sheath cell IFL
mCherry c264Tg; nksagff214aGt; pd1151Tg (EKW) standard conditions Long-pec notochord inner cell IFL
Protruding-mouth notochord inner cell IFL
c264Tg; nksagff214aGt; pd1151Tg (EKW) chemical treatment by environment: metronidazole Long-pec notochord inner cell IFL
Protruding-mouth notochord inner cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
c264Tg; nksagff214aGt; pd1151Tg (EKW) chemical treatment by environment: metronidazole Long-pec notochord inner cell dead, abnormal
Long-pec notochord outer sheath cell displaced to notochord central region, abnormal
Protruding-mouth notochord inner cell dead, abnormal
Protruding-mouth notochord outer sheath cell displaced to notochord central region, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.