ZFIN ID: ZDB-FIG-170815-8 |
Genes: | |
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Fish: | |
Condition: | |
Anatomical Terms: | |
Stage Range: | Long-pec to Protruding-mouth |
Fish: | |
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Condition: | |
Observed In: | |
Stage Range: | Long-pec to Protruding-mouth |
Fig. 3 Release of Vacuolated Cell Contents Triggers Sheath Cell Invasion (A and B) Confocal images of live WT embryos expressing sag:gal4::UAS:mcherry-NTR in vacuolated cells and col9a2:GFPCaaX in sheath cells. (C and D) In embryos treated with 1.5 mM metronidazole (MTZ), sheath cells invade areas of vacuolated cell death. Arrows point to invading sheath cells; asterisks mark intact vacuolated cells. (E) LC-MS quantitation of ATP and UTP levels in nucleus pulposus (NP) and annulus fibrosis (AF) tissue isolated from 6-month-old pig spines. n = 12 for NP and 4 for AF. ∗∗∗p < 0.001, Student’s t test. The scale bars represent 100 μm. Error bars are SD. See also Figures S3 and S4. |
Acknowledgments: | ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol. |
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