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ZFIN ID: ZDB-FIG-170815-8
Garcia et al., 2017 - Sheath Cell Invasion and Trans-differentiation Repair Mechanical Damage Caused by Loss of Caveolae in the Zebrafish Notochord. Current biology : CB   27(13):1982-1989.e3 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

Release of Vacuolated Cell Contents Triggers Sheath Cell Invasion

(A and B) Confocal images of live WT embryos expressing sag:gal4::UAS:mcherry-NTR in vacuolated cells and col9a2:GFPCaaX in sheath cells.

(C and D) In embryos treated with 1.5 mM metronidazole (MTZ), sheath cells invade areas of vacuolated cell death. Arrows point to invading sheath cells; asterisks mark intact vacuolated cells.

(E) LC-MS quantitation of ATP and UTP levels in nucleus pulposus (NP) and annulus fibrosis (AF) tissue isolated from 6-month-old pig spines. n = 12 for NP and 4 for AF. ∗∗∗p < 0.001, Student’s t test.

The scale bars represent 100 μm. Error bars are SD. See also Figures S3 and S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP c264Tg ; nksagff214aGt ; pd1151Tg(EKW) standard conditions Long-pec notochord outer sheath cell IFL
Protruding-mouth notochord outer sheath cell IFL
c264Tg ; nksagff214aGt ; pd1151Tg(EKW) chemical treatment by environment: metronidazole Long-pec notochord outer sheath cell IFL
Protruding-mouth notochord outer sheath cell IFL
mCherry c264Tg ; nksagff214aGt ; pd1151Tg(EKW) standard conditions Long-pec notochord inner cell IFL
Protruding-mouth notochord inner cell IFL
c264Tg ; nksagff214aGt ; pd1151Tg(EKW) chemical treatment by environment: metronidazole Long-pec notochord inner cell IFL
Protruding-mouth notochord inner cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
c264Tg ; nksagff214aGt ; pd1151Tg(EKW) chemical treatment by environment: metronidazole Long-pec notochord inner cell dead, abnormal
Long-pec notochord outer sheath cell displaced to notochord central region, abnormal
Protruding-mouth notochord inner cell dead, abnormal
Protruding-mouth notochord outer sheath cell displaced to notochord central region, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.