FIGURE

Fig. 3

ID

ZDB-FIG-170815-8

Publication

Garcia et al., 2017 - Sheath Cell Invasion and Trans-differentiation Repair Mechanical Damage Caused by Loss of Caveolae in the Zebrafish Notochord

Other Figures

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Fig. 3

Release of Vacuolated Cell Contents Triggers Sheath Cell Invasion

(A and B) Confocal images of live WT embryos expressing sag:gal4::UAS:mcherry-NTR in vacuolated cells and col9a2:GFPCaaX in sheath cells.

(C and D) In embryos treated with 1.5 mM metronidazole (MTZ), sheath cells invade areas of vacuolated cell death. Arrows point to invading sheath cells; asterisks mark intact vacuolated cells.

(E) LC-MS quantitation of ATP and UTP levels in nucleus pulposus (NP) and annulus fibrosis (AF) tissue isolated from 6-month-old pig spines. n = 12 for NP and 4 for AF. ^∗∗∗p < 0.001, Student’s t test.

The scale bars represent 100 μm. Error bars are SD. See also Figures S3 and S4.

Expression Data

Genes:	GFP mCherry
Fish:	c264Tg; nksagff214aGt; pd1151Tg (EKW)
Condition:	chemical treatment by environment: metronidazole
Anatomical Terms:	notochord inner cell notochord outer sheath cell
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	c264Tg; nksagff214aGt; pd1151Tg (EKW)
Condition:	chemical treatment by environment: metronidazole
Observed In:	notochord central region notochord inner cell notochord outer sheath cell
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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