ZFIN Figure: Garcia et al., 2017, Fig. 3

ZFIN ID: ZDB-FIG-170815-8

ZFIN ID: ZDB-FIG-170815-8

Garcia et al., 2017 - Sheath Cell Invasion and Trans-differentiation Repair Mechanical Damage Caused by Loss of Caveolae in the Zebrafish Notochord. Current biology : CB 27(13):1982-1989.e3 Full text @ Curr. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	GFP mCherry
Fish:	c264Tg ; nksagff214aGt ; pd1151Tg(EKW)
Condition:	chemical treatment by environment: metronidazole
Anatomical Terms:	notochord inner cell notochord outer sheath cell
Stage Range:	Long-pec to Protruding-mouth

PHENOTYPE:

Fish:	c264Tg ; nksagff214aGt ; pd1151Tg(EKW)
Condition:	chemical treatment by environment: metronidazole
Observed In:	notochord central region notochord inner cell notochord outer sheath cell
Stage Range:	Long-pec to Protruding-mouth

Fig. 3

Release of Vacuolated Cell Contents Triggers Sheath Cell Invasion

(A and B) Confocal images of live WT embryos expressing sag:gal4::UAS:mcherry-NTR in vacuolated cells and col9a2:GFPCaaX in sheath cells.

(C and D) In embryos treated with 1.5 mM metronidazole (MTZ), sheath cells invade areas of vacuolated cell death. Arrows point to invading sheath cells; asterisks mark intact vacuolated cells.

(E) LC-MS quantitation of ATP and UTP levels in nucleus pulposus (NP) and annulus fibrosis (AF) tissue isolated from 6-month-old pig spines. n = 12 for NP and 4 for AF. ^∗∗∗p < 0.001, Student’s t test.

The scale bars represent 100 μm. Error bars are SD. See also Figures S3 and S4.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
GFP	c264Tg ; nksagff214aGt ; pd1151Tg(EKW)	standard conditions	Long-pec	notochord outer sheath cell	IFL
			Protruding-mouth	notochord outer sheath cell	IFL
	c264Tg ; nksagff214aGt ; pd1151Tg(EKW)	chemical treatment by environment: metronidazole	Long-pec	notochord outer sheath cell	IFL
			Protruding-mouth	notochord outer sheath cell	IFL
mCherry	c264Tg ; nksagff214aGt ; pd1151Tg(EKW)	standard conditions	Long-pec	notochord inner cell	IFL
			Protruding-mouth	notochord inner cell	IFL
	c264Tg ; nksagff214aGt ; pd1151Tg(EKW)	chemical treatment by environment: metronidazole	Long-pec	notochord inner cell	IFL
			Protruding-mouth	notochord inner cell	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
c264Tg ; nksagff214aGt ; pd1151Tg(EKW)	chemical treatment by environment: metronidazole	Long-pec	notochord inner cell dead, abnormal
		Long-pec	notochord outer sheath cell displaced to notochord central region, abnormal
		Protruding-mouth	notochord inner cell dead, abnormal
		Protruding-mouth	notochord outer sheath cell displaced to notochord central region, abnormal

Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.