ZFIN Figure: Garcia et al., 2017, Fig. 3

ZFIN ID: ZDB-FIG-170815-8

ZFIN ID: ZDB-FIG-170815-8

Garcia et al., 2017 - Sheath Cell Invasion and Trans-differentiation Repair Mechanical Damage Caused by Loss of Caveolae in the Zebrafish Notochord. Current biology : CB 27(13):1982-1989.e3 Full text @ Curr. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	GFP mCherry
Fish:	c264Tg; nksagff214aGt; pd1151Tg (EKW)
Condition:	chemical treatment by environment: metronidazole
Anatomical Terms:	notochord inner cell notochord outer sheath cell
Stage Range:	Long-pec to Protruding-mouth

PHENOTYPE:

Fish:	c264Tg; nksagff214aGt; pd1151Tg (EKW)
Condition:	chemical treatment by environment: metronidazole
Observed In:	notochord central region notochord inner cell notochord outer sheath cell
Stage Range:	Long-pec to Protruding-mouth

Fig. 3

Release of Vacuolated Cell Contents Triggers Sheath Cell Invasion

(A and B) Confocal images of live WT embryos expressing sag:gal4::UAS:mcherry-NTR in vacuolated cells and col9a2:GFPCaaX in sheath cells.

(C and D) In embryos treated with 1.5 mM metronidazole (MTZ), sheath cells invade areas of vacuolated cell death. Arrows point to invading sheath cells; asterisks mark intact vacuolated cells.

(E) LC-MS quantitation of ATP and UTP levels in nucleus pulposus (NP) and annulus fibrosis (AF) tissue isolated from 6-month-old pig spines. n = 12 for NP and 4 for AF. ^∗∗∗p < 0.001, Student’s t test.

The scale bars represent 100 μm. Error bars are SD. See also Figures S3 and S4.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
GFP	c264Tg; nksagff214aGt; pd1151Tg (EKW)	standard conditions	Long-pec	notochord outer sheath cell	IFL
			Protruding-mouth	notochord outer sheath cell	IFL
	c264Tg; nksagff214aGt; pd1151Tg (EKW)	chemical treatment by environment: metronidazole	Long-pec	notochord outer sheath cell	IFL
			Protruding-mouth	notochord outer sheath cell	IFL
mCherry	c264Tg; nksagff214aGt; pd1151Tg (EKW)	standard conditions	Long-pec	notochord inner cell	IFL
			Protruding-mouth	notochord inner cell	IFL
	c264Tg; nksagff214aGt; pd1151Tg (EKW)	chemical treatment by environment: metronidazole	Long-pec	notochord inner cell	IFL
			Protruding-mouth	notochord inner cell	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
c264Tg; nksagff214aGt; pd1151Tg (EKW)	chemical treatment by environment: metronidazole	Long-pec	notochord inner cell dead, abnormal
		Long-pec	notochord outer sheath cell displaced to notochord central region, abnormal
		Protruding-mouth	notochord inner cell dead, abnormal
		Protruding-mouth	notochord outer sheath cell displaced to notochord central region, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.