ZFIN ID: ZDB-IMAGE-170815-3
Figures for Garcia et al., 2017

Genes:

Figure Caption/Comments:

Fig. 3

Release of Vacuolated Cell Contents Triggers Sheath Cell Invasion

(A and B) Confocal images of live WT embryos expressing sag:gal4::UAS:mcherry-NTR in vacuolated cells and col9a2:GFPCaaX in sheath cells.

(C and D) In embryos treated with 1.5 mM metronidazole (MTZ), sheath cells invade areas of vacuolated cell death. Arrows point to invading sheath cells; asterisks mark intact vacuolated cells.

(E) LC-MS quantitation of ATP and UTP levels in nucleus pulposus (NP) and annulus fibrosis (AF) tissue isolated from 6-month-old pig spines. n = 12 for NP and 4 for AF. ∗∗∗p < 0.001, Student’s t test.

The scale bars represent 100 μm. Error bars are SD. See also Figures S3 and S4.

Figure Data:
Acknowledgments:
ZFIN wishes to thank the journal for permission to reproduce figures from this article. Please note that this material may be protected by copyright.