FIGURE

Fig. 6

ID

ZDB-FIG-170815-40

Publication

Lin et al., 2017 - microRNA-206 modulates an Rtn4a/Cxcr4a/Thbs3a axis in newly forming somites to maintain and stabilize the somite boundary formation of zebrafish embryos.

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Fig. 6

Change of expression levels of miR-206, Rtn4al, Cxcr4a or Thbs3a fails to epithelialize somites in zebrafish embryos. (a) A diagram depicts that centrosomes of epithelial cells at the somite boundary are localized apically when epithelial cells undergo MET. (b) WT embryos at 20 hpf; (c) knockdown of miR-206, (d) overexpression of rtn4al, (e) knockdown of cxcr4a and (f) overexpression of thbs3a. Fibronectin labelled with green fluorescent signal was used to mark the somite boundary; Phalloidin labelled with red fluorescent signal was used to mark F-actin, while γ-tubulin labelled with blue fluorescent signal was used to mark centrosomes. (b–f) Three fluorescent signals were merged; (b′–f′) were amplified from the corresponding panels (b–f). White arrowheads indicate centrosomes not localized apically in the epithelial cells of the defective somite boundary.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-cxcr4a AB + MO3-mir206-2
Knockdown Reagents:	MO2-cxcr4a MO3-mir206-2
Observed In:	somite border centrosome somite border epithelial cell somite border mesenchymal to epithelial transition
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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