FIGURE

Fig. 5

ID

ZDB-FIG-170809-6

Publication

Mesureur et al., 2017 - Macrophages, but not neutrophils, are critical for proliferation of Burkholderia cenocepacia and ensuing host-damaging inflammation

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Fig. 5

Neutrophils efficiently phagocytose surface-associated Bcc bacteria.

Confocal stacks after subcutaneous infection with B. stabilis in mpx:GFP embryos (STD intensity projection, 2 μm x 19 steps; T = 35–37 in S2 Movie). Arrows, rounded neutrophils (green) with vacuoles full of bacteria (red) at 64 minutes post injection (mpi) eject their cell contents in the surroundings (arrow heads 65.5 mpi), leaving bacterial clusters and cell debris (arrow heads, 66 min). Lower panels, MAX intensity projection of three consecutive slices (2 μm) at 65.5 mpi showing ejected cellular contents (diffuse GFP signal). Scale bars, 50 μm.

Expression Data

Gene:	GFP
Fish:	i114Tg
Condition:	bacterial treatment by injection: Burkholderia stabilis
Anatomical Term:	neutrophil
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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